Motivation and Goals of this Position
The bacterium E. coli is a very prominent host for producing recombinant proteins for biopharmaceuticals. Often, the product is accumulated as Inclusions Bodies (IBs) inside of the cell. In order to recover the product, the IB is processed in a process chain: solubilized, refolded and concentrated. So far, this process is prone to very high losses in product yield. In the last two years we earned lots of experience in analyzing the effect of process parameters on the refolding step yield.
The next consequent step and goal of this PhD position is the development of a continuous microbial down stream process around protein refolding. This includes analytical resolution of refolding dynamics and advanced experimental planning, execution and data analysis. This PhD position focusses on the development of the process chain at laboratory scale. The position is tightly linked to the second PhD working on the development of Digital Twins for this process.
This PhD position runs within a CHASE Competence Centre and is executed at the TU WIEN.
- Master of Science in Bioprocess Technology, Biotechnology, Electrical Engineering, Chemical Engineering or similar.
- Experience in analytical methods (HPLC, spectroscopic methods) and background in bioprocess technology
- Experience in operating bioreactors and downstream processing in laboratory scale
- Experience in protein refolding from inclusion bodies would be a plus
- Sensitivity for data analysis and statistical assessment of large data sets
- A superior command of English
- Ability to work in a team
- You should be accustomed to networked critical analytical thinking, scientifically interested and able to work in a team respecting tight project timelines.
We offer a highly interesting, diversified position comprising bioprocess technology projected on circular economy bioprocesses in tight cooperation applied basic science projects with industrial partners.
This PhD position starts on October 1st 2020 and is scheduled for 3 years. The monthly minimum wage is currently € 2.148, 00 (14x per year), before tax, at a 30h/week employment.
The TU Wien aims to increase the proportion of women and encourages qualified women to apply.